The Reaction of Iodoacetate with Ribonuclease-S
نویسندگان
چکیده
منابع مشابه
The reaction of iodoacetate with ribonuclease-S.
The reaction of iodoacetic acid with ribonuclease has been studied recently by Gundlach et al. (1). Inactivation of the enzyme occurred over a wide range of pH. The authors concluded that at acid pH the reaction involved solely methionyl residues, that at pH 5.5 a histidyl residue was the principal site of attack, and that at alkaline pH values significant reaction at lysyl residues occurred. O...
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lodoacetic acid and its amide have been widely used in protein chemistry as reagents for sulfhydryl groups. Although both can be expected to undergo other reactions with proteins, for example, with the e-amino groups of lysine residues, the imidazole groups of histidine residues, and the phenolic hydroxyls of tyrosine residues, these reactions arc generally slower than is the one with sulfhydry...
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The reaction of fumarase with several alkylating agents has been examined. The structural similarity between these compounds and the natural substrates for the enzyme, fumarate and malate, suggested that reaction might occur with structures in the active sites. Of all the compounds tested, only iodoacetate and 4-bromocrotonate inactivated fumarase by modification of structures other than the th...
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During recent years, successful efforts have been made to modify a number of enzymes by specific chemical reactions that result in loss of activity. In this way progress is being made toward locating the group of amino acid residues (so called “active center”) that participates in the catalytic action. The organic esters of phosphoric acid, such as diisopropylphosphorofluoridate, have been part...
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The iodoacetate-nitrogen-poisoned muscle offers the possibility of studying the stoichiometry of the single muscle twitch since metabolic resynthesis by glycolysis and oxidative phosphorylation are blocked, and there remains as an energy source only the creatine phosphoryltransfer system, creatine phosphate reacting with adenosinediphosphate to give the triphosphate and creatine. It is shown, p...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1961
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)64182-1